CRISPR sgRNA Cloning & Control Vectors

CRISPR sgRNA cloning vectors and control constructs for individual gene-editing experiments

When you need individual sgRNA expression vectors and matched controls for one-off gene-editing rather than pooled screens, this section covers the vectors. Empty sgRNA vectors with type-II restriction sites for guide insertion, scrambled and non-targeting controls, validated positive controls, and dual-guide expression backbones for paired-cut strategies.

Pick by backbone and integration.

1. Backbone. Lentiviral for stable integration. Plasmid for transient plus selection. AAV for in vivo delivery.
2. Promoter. U6 standard for sgRNA. H1 or T7 in specialised systems.
3. All-in-one versus split. Cas9-and-sgRNA on one vector saves transfection logistics. Split vectors enable independent timing or dose.
4. Reporter or selection. Puromycin standard. GFP for FACS sorting. Combined for stringent selection.
5. Controls. Always include scrambled and non-targeting controls in early experiments.

OriGene and Cellecta supply individual cloning vectors and matched controls.