CRISPR/Cas9 Safe Harbor Knock-in

CRISPR/Cas9 safe-harbour knock-in for predictable, single-copy transgene expression

When you need predictable, single-copy transgene expression rather than random lentiviral integration, safe-harbour knock-in delivers it. CRISPR/Cas9 homology-directed repair at characterised loci (AAVS1, ROSA26, hH11, CCR5) gives stable expression without position-effect variability.

Pick locus and donor.

1. Safe harbour locus. AAVS1 is the human gold standard for transgene expression stability. ROSA26 is the mouse equivalent. CCR5 has additional functional benefit in HIV research.
2. Donor design. Homology arms length and composition. Promoter and selectable marker matched to your downstream selection.
3. Validation evidence. Verify the vector has published demonstration of single-copy integration efficiency in your cell type.
4. Downstream selection. Puromycin, neomycin, blasticidin, or fluorescent reporter. Match to your selection scheme.
5. Verification. Single-copy integration verification by ddPCR or Southern blot is essential for downstream quantitative work.

OriGene leads with AAVS1 and ROSA26 donor vector systems.