Cold Fusion Cloning Kits

Cold Fusion kits for seamless, ligase-free multi-fragment DNA cloning in hours

When you need seamless multi-fragment cloning without restriction enzymes or ligase, Cold Fusion joins linear DNA fragments through short overlapping sequences. The chemistry compresses cloning workflows from days to hours.

Pick by fragment count.

1. Two-fragment joining. Most Cold Fusion kits optimise for this.
2. Multi-fragment assembly. Some kits handle 4 to 6 fragments in one reaction. Verify joining efficiency on a benchmark fragment set before committing to a critical construct.
3. Chemistry. Isothermal incubation versus structured-cycle protocols.
4. Insert size flexibility. Verify across your size range.
5. Validation. Sanger-sequence assembled constructs before downstream use.

SBI System Biosciences supplies Cold Fusion kits.