EpiDyne nucleosome substrates for studying ATP-dependent chromatin remodelling enzyme activity
When you study ATP-dependent chromatin remodelling enzymes (SWI/SNF, ISWI, CHD, INO80) and need a defined nucleosome substrate, EpiDyne pairs position-defined nucleosomes on extended DNA scaffolds. Restriction-enzyme accessibility or fluorescence-based readouts capture sliding, ejection, or assembly activity.
Pick by readout.
- Sliding. Restriction enzyme accessibility assay.
- Kinetic monitoring. Fluorescence-quench or FRET.
- Compositional changes. Mass spectrometry.
- Positioning fidelity. Uniquely positioned 601-sequence substrates give cleaner kinetic data than randomly positioned arrays.
- High-throughput drug screening. Use homogeneous fluorescence-based readouts. For mechanistic work use restriction-enzyme-accessibility assays to confirm nucleosome positioning changes.
EpiCypher supplies the EpiDyne platform.
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