Chromatin Preparation & Analysis

Chromatin preparation reagents for nuclei isolation, shearing, and quality control upstream of ChIP

When you need general chromatin preparation upstream of ChIP, CUT&RUN, or chromatin profiling, this section covers nuclei isolation, chromatin shearing (sonication and enzymatic), and quality QC tools. Quality of upstream chromatin prep directly drives downstream IP success.

Pick by shearing and input.

1. Shearing method. Sonication for high-input flexibility. MNase-based for sharper fragment-size distribution and lower-input compatibility.
2. Quality control. Some kits include built-in fragment-size verification.
3. Input flexibility. Match kit to your starting cell number.
4. Use case. For low-input chromatin profiling MNase outperforms sonication. For high-input legacy workflows sonication remains standard.

EpigenTek supplies chromatin prep reagents.