Chromatin Loop Formation (dCas9)

dCas9-based tools for inducible chromatin loop formation with time-resolved control

When you want to study enhancer-promoter loop formation or 3D-genome perturbation mechanistically, dCas9-based tools induce artificial chromatin loops between defined regions through dimerisation domains. Inducible chemistry gives time-resolved control over loop formation.

Pick by temporal control.

1. Inducible. Choose chemical-inducible (rapamycin-FKBP-FRB) or light-inducible (Cry2-CIB1) dimerisation for time-resolved studies.
2. Constitutive. For steady-state perturbation experiments.
3. dCas9 variant. High-fidelity dCas9 minimises off-target binding.
4. Validation. Confirm dCas9 binding by ChIP. Validate loop formation by Capture Hi-C or 3C-PCR.
5. Off-target characterisation. Important before drawing mechanistic conclusions.

SBI System Biosciences supplies the tools in this inventory.