Cell Tracking by NGS Barcode Labelling

Lentiviral DNA barcode libraries for clonal lineage tracing and cell tracking by sequencing

When you need to track many cell clones in parallel through proliferation, fate, or competitive growth, lentiviral barcode libraries label each cell with a unique DNA sequence readable by sequencing. The technology supports lineage tracing, clonal dynamics in xenografts and metastasis models, immune cell tracking, and pooled competitive growth assays.

Pick by complexity and resolution.

1. Barcode complexity. For low-frequency clonal events match library complexity to at least 10-fold above the cells barcoded. Singly-barcoded cells must dominate the pool.
2. Readout. Bulk NGS for population-level dynamics. Single-cell barcode readout for clone-level lineage trees.
3. Reporter integration. Barcode-only versus barcode-plus-fluorescent-reporter for FACS isolation of specific clones.
4. Computational pipeline. Verify supplier pipeline matches your bioinformatics setup.
5. Cell-input scaling. Scale barcoded cells with library complexity.

Cellecta leads with LARRY and pooled barcode platforms.