Cell Signaling

Cell signalling reagents for measuring and modulating major mammalian pathways

When you need to measure or modulate canonical mammalian signalling pathways (MAPK, PI3K/AKT, NF-kB, Wnt, Hedgehog, Notch), this section covers the reagents. Pathway-specific reporter constructs, small-molecule modulators, signaling protein recombinants, and pathway-readout ELISAs cover the major pathways.

Pick by pathway and readout.

1. Pathway. Each major pathway has specific reporters, antibodies, and pharmacological tools.
2. Readout. Transcriptional reporter for chronic pathway state. ELISA or Western for protein-modification snapshot. Kinase activity assay for direct enzyme readout.
3. Context. Cell-line versus primary cell versus in vivo each have different best-fit tools.
4. Orthogonal confirmation. Pathway-perturbation studies benefit from confirming with two independent readouts.
5. Specificity. Confirm reporter-based pathway activation with a direct biochemical readout (Western, ELISA, kinase assay) for mechanism-of-action work.

GeneCopoeia leads pathway-specific transcriptional reporters. Cell Biolabs supplies pathway ELISAs and small-molecule modulators.