Cas9 & dCas9 Expression Constructs

Cas9, dCas9, and base editor expression constructs for gene editing and regulation

When you need Cas9 for editing, dCas9 for activating or repressing genes, base editors for precision SNV changes, or paired guide expression for nickase strategies, this section covers the expression constructs. Lentiviral, plasmid, and AAV backbones plus promoters (CMV, EF1-alpha, tissue-specific, inducible) cover the range.

Pick effector type and delivery.

1. Effector. Cas9 for knockout. dCas9-VPR or dCas9-VP64 for CRISPRa activation. dCas9-KRAB-MeCP2 for CRISPRi repression. CBE or ABE for base editing. Prime editor for templated edits.
2. Delivery. Lentiviral for stable. Plasmid for transient. AAV for in vivo.
3. Promoter. CMV or EF1-alpha for constitutive. Tet-on for dox-inducible. Tissue-specific for targeted expression.
4. Selection. Puromycin, blasticidin, hygromycin, or fluorescent reporters. Match to your selection scheme.
5. Validation. Verify editing efficiency in your cell type before scaling.

ABM and Cellecta supply broad construct catalogues. ABM covers all-in-one sgRNA-plus-Cas9 vectors. Cellecta focuses on pooled library backbones.